On Tue, 7 Sep 2004 05:02:00 +0000 (UTC), 
Michael Ragland  wrote:

[snip]

> Re: What is the purpose of chromosome walking?
> 
> Date: Mon Dec 6 13:09:41 1999
> Posted By: Christopher Carlson, Grad student Genetics
> Area of science: Molecular Biology
> ID: 944432152.Mb 

[snip]

> To put this in historical perspective, the first major use of 
> chromosome walking was to find the Cystic fibrosis gene, all the way 
> back in 1989.
> http://www3.ncbi.nlm.nih.gov/htbin-post/Omim/dispmim?602421#CLONING 

One of the most famous examples of the technique involved the isolation
of the bithorax (HOX) genes in 1983.

    Bender, W., Spierer, P., and Hogness, D.S. (1983) Chromosomal 
         walking and jumping to isolate DNA from the Ace and rosy 
         loci and the bithorax complex in Drosophila melanogaster.
         J. Mol. Biol. 168:17-33.

This was not the first use of the technique. It was used earlier in
cloning bacteriophage lambda and E. coli genes (among others).



Larry Moran
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